人蛋白二硫化物异构酶A3(PDIA3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10355
- Price：Negotiable
- Clicks：1641
Product Detail
Product Information
| Product Name | Human PDIA3(Protein Disulfide Isomerase A3) ELISA Kit |
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| Alternative Names | P58; ERp61; ERp57; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein,58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.46 ng/mL |
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| Detection Range | 1.56-100 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Enzyme & Kinase;Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P30101 |
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Standard Curve
| Standard Curve |  |
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