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兔基质金属蛋白酶2(MMP2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10340
  • Price:Negotiable
  • Clicks:2144

Product Detail

Product Information

Product NameRabbit MMP2(Matrix Metalloproteinase 2) ELISA Kit
Alternative NamesCLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

Assay Parameters

SpeciesRabbit
Assay TypeSandwich
Sensitivity0.54 ng/mL
Detection Range1.56-100 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP50757

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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