人脱氧核糖核酸酶Ⅰ(DNASE1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10328
- Price：Negotiable
- Clicks：1575
Product Detail
Product Information
| Product Name | Human DNASE1(Deoxyribonuclease I) ELISA Kit |
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| Alternative Names | DNaseI; DNL1; DRNI; DNase-I; Dornase alfa |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.098 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | Serum, plasma, urine, saliva, seminal plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase;Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNASE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNASE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNASE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNASE1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P24855 |
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Standard Curve
| Standard Curve |  |
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