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小鼠血清淀粉样蛋白A(SAA)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10304
  • Price:Negotiable
  • Clicks:1681

Product Detail

Product Information

Product NameMouse SAA(Serum Amyloid A) ELISA Kit
Alternative NamesSA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity2.67 ng/mL
Detection Range7.81-500 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;Kidney biomarker;Hepatology;Urology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SAA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP05366

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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