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大鼠血管紧张素Ⅰ(AngI)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10303
  • Price:Negotiable
  • Clicks:2081

Product Detail

Product Information

Product NameRat AngI(Angiotensin I) ELISA Kit
Alternative NamesAng-I; Angiotensin-1

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.059 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaEndocrinology;Cardiovascular biology;Hepatology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AngI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AngI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AngI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AngI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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