人凝血因子Ⅸ(F9)酶联免疫吸附检测试剂盒

Product Name	Human F9(Coagulation Factor IX) ELISA Kit
Alternative Names	HEMB; FIX; GLA Domain; PTC; Anti Hemophilic Factor B; Christmas Factor; Plasma Thromboplastic Component; Christmas Disease; Hemophilia B

Research Area	Signal transduction;Hematology;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F9 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P00740

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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