大鼠组织金属蛋白酶抑制因子1(TIMP1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10237
- Price：Negotiable
- Clicks：2005
Product Detail
Product Information
| Product Name | Rat TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit |
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| Alternative Names | EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 1.41 ng/mL |
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| Detection Range | 3.13-200 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P30120 |
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Standard Curve
| Standard Curve |  |
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