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人簇集蛋白(CLU)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10192
  • Price:Negotiable
  • Clicks:1590

Product Detail

Product Information

Product NameHuman CLU(Clusterin) ELISA Kit
Alternative NamesCLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.57 µg/mL
Detection Range1.57-100 µg/mL
Sample Typeserum, plasma, urine, saliva, tissue homogenates, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaApoptosis;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO75153

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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