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人乳铁传递蛋白(LTF)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10188MS
  • Price:Negotiable
  • Clicks:2482

Product Detail

Product Information

Product NameHuman LTF(Lactoferrin) Microsample ELISA Kit
Alternative NamesHLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity21.3 ng/mL
Detection Range78.13-5000 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP02788

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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