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小鼠C反应蛋白(CRP)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10186MS
  • Price:Negotiable
  • Clicks:2291

Product Detail

Product Information

Product NameMouse CRP(C Reactive Protein) Microsample ELISA Kit
Alternative NamesC-RP; PTX1; Pentraxin-Related; Pentraxin 1

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity1.09 µg/mL
Detection Range1.56-100 µg/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP14847

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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