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大鼠高敏C反应蛋白(hs-CRP)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10185MS
  • Price:Negotiable
  • Clicks:2448

Product Detail

Product Information

Product NameRat hs-CRP(High Sensitivity C Reactive Protein) Microsample ELISA Kit
Alternative NamesC-RP; PTX1; Pentraxin-Related; Pentraxin 1

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity1.08 µg/mL
Detection Range1.56-100 µg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP48199

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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