小鼠前颗粒蛋白(PGRN)酶联免疫吸附检测试剂盒

Product Name	Mouse PGRN(Progranulin) ELISA Kit
Alternative Names	PGRN; Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; Proepithelin

Species	Mouse
Assay Type	Sandwich
Sensitivity	0.41 ng/mL
Detection Range	1.56-100 ng/mL
Sample Type	Serum, plasma and other biological fluids.
Assay Length	3.5h

Research Area	Cytokine
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PGRN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P28798

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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