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大鼠核心蛋白聚糖(DCN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10156
  • Price:Negotiable
  • Clicks:1460

Product Detail

Product Information

Product NameRat DCN(Decorin) ELISA Kit
Alternative NamesCSCD; DSPG2; PG40; PGII; PGS2; PG-S2; SLRR1B; Bone proteoglycan II

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity6.12 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Developmental science;Bone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DCN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DCN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DCN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DCN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ01129

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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