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小鼠神经细胞黏附分子(NCAM)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10128
  • Price:Negotiable
  • Clicks:1799

Product Detail

Product Information

Product NameMouse NCAM(Neural Cell Adhesion Molecule) ELISA Kit
Alternative NamesCD56; NCAM1; MSK39; N-CAM-1

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity1.22 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;Tumor immunity;Neuro science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NCAM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP13595

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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