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犬胱抑素C(Cys-C)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10127
  • Price:Negotiable
  • Clicks:2256

Product Detail

Product Information

Product NameDog Cys-C(Cystatin C) ELISA Kit
Alternative NamesCST3; Cystatin 3; ARMD11; Post-Gamma-Globulin

Assay Parameters

SpeciesDog
Assay TypeSandwich
Sensitivity0.64 ng/mL
Detection Range1.56-100 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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