| Product Name | Dog Cys-C(Cystatin C) ELISA Kit |
|---|---|
| Alternative Names | CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin |
| Species | Dog |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 0.64 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve. |
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