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山羊雌二醇(E2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10122
  • Price:Negotiable
  • Clicks:2488

Product Detail

Product Information

Product NameGoat E2(Estradiol) ELISA Kit
Alternative Names17B-Estradiol; Oestradiol; Beta-Estradiol

Assay Parameters

SpeciesGoat
Assay TypeCompetitive Inhibition
Sensitivity14.45 pg/mL
Detection Range46.88-3000 pg/mL
Sample Typeserum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length2h

Research Information

Research AreaEndocrinology;Reproductive science;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Goat E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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