斑马鱼免疫球蛋白M(IgM)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10111
- Price：Negotiable
- Clicks：2285
Product Detail
Product Information
| Product Name | Zebrafish IgM(Immunoglobulin M) ELISA Kit |
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| Alternative Names | IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region |
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Assay Parameters
| Species | Zebrafish |
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| Assay Type | Sandwich |
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| Sensitivity | 14.3 µg/mL |
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| Detection Range | 31.25-2000 µg/mL |
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| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Infection immunity;Immune molecule;Hematology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish IgM in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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