猪胶质细胞系来源神经营养因子(GDNF)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10092
- Price：Negotiable
- Clicks：2372
Product Detail
Product Information
| Product Name | Pig GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit |
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| Alternative Names | ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor |
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Assay Parameters
| Species | Pig |
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| Assay Type | Sandwich |
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| Sensitivity | 0.058 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Cytokine;Apoptosis;Neuro science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig GDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig GDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig GDNF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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