| Product Name | Horse ALB(Albumin) ELISA Kit |
|---|---|
| Alternative Names | Serum albumin |
| Species | Horse |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 0.49 µg/mL |
| Detection Range | 1.57-100 µg/mL |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
| Research Area | Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse ALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ALB in the samples is then determined by comparing the OD of the samples to the standard curve. |