大鼠肝细胞核因子1α(HNF1a)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK10044MS
- Price：Negotiable
- Clicks：1995
Product Detail
Product Information
| Product Name | Rat HNF1a(Hepatocyte Nuclear Factor 1 Alpha) Microsample ELISA Kit |
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| Alternative Names | TCF1; HNF1; LFB1; LF-B1; MODY3; Transcription Factor 1,Hepatic; Hepatic Nuclear Factor; Albumin Proximal Factor; Liver-specific transcription factor LF-B1 |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.052 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Cytokine; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNF1a in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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