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大鼠对称性二甲基精氨酸(SDMA)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10039
  • Price:Negotiable
  • Clicks:1578

Product Detail

Product Information

Product NameRat SDMA(Symmetric dimethylarginine) ELISA Kit
Alternative NamesSymmetric dimethylarginine

Assay Parameters

SpeciesRat
Assay TypeCompetitive Inhibition
Sensitivity0.049 ng/mL
Detection Range0.16-10 ng/mL
Sample Typecell lysates, cell culture supernates and other biological fluids
Assay Length2h

Research Information

Research AreaSignal transduction;Developmental science;Bone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SDMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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