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人前列腺素F2α(PGF2a)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10032
  • Price:Negotiable
  • Clicks:1975

Product Detail

Product Information

Product NameHuman PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Alternative NamesPGF2A; PGF2-A; PG-F2a

Assay Parameters

SpeciesHuman
Assay TypeCompetitive Inhibition
Sensitivity3.65 pg/mL
Detection Range12.5-800 pg/mL
Sample TypeSerum, plasma, saliva and other biological fluids
Assay Length2h

Research Information

Research AreaMetabolic pathway;Cardiovascular biology;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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