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犬Ⅱ型胶原交联羧基端肽(CTXII)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10021
  • Price:Negotiable
  • Clicks:1513

Product Detail

Product Information

Product NameDog CTXII(Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit
Alternative NamesCTX-II; CTX2

Assay Parameters

SpeciesDog
Assay TypeCompetitive Inhibition
Sensitivity53.5 pg/mL
Detection Range156.25-10000 pg/mL
Sample Typeserum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length2h

Research Information

Research AreaMetabolic pathway;Bone metabolism;Rheumatology;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CTXII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CTXII in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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