大鼠线粒体丝氨酸/苏氨酸蛋白激酶PINK1(PINK1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK10019
- Price：Negotiable
- Clicks：2302
Product Detail
Product Information
| Product Name | Rat PINK1(Serine/threonine-protein kinase PINK1, mitochondrial) ELISA Kit |
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| Alternative Names | PTEN-induced putative kinase protein 1; BRPK |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.14 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PINK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PINK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PINK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PINK1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | B5DFG1 |
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Standard Curve
| Standard Curve |  |
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