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牛含铜胺氧化酶3(AOC3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK10017
  • Price:Negotiable
  • Clicks:1849

Product Detail

Product Information

Product NameCattle AOC3(Amine Oxidase, Copper Containing 3) ELISA Kit
Alternative NamesVAP1; HPAO; SSAO; Membrane primary amine oxidase; Vascular Adhesion Protein 1; Semicarbazide-Sensitive Amine Oxidase; Copper-Containing Amine Oxygen Oxidoreductase

Assay Parameters

SpeciesCattle
Assay TypeSandwich
Sensitivity6.9 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Infection immunity;Endocrinology;Bone metabolism;Rheumatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle AOC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle AOC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle AOC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle AOC3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9TTK6

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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