小鼠S-期激酶关联蛋白1(SKP1)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK0991MS
- Price：Negotiable
- Clicks：1731
Product Detail
Product Information
| Product Name | Mouse SKP1(S-Phase Kinase Associated Protein 1) Microsample ELISA Kit |
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| Alternative Names | SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.056 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SKP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SKP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SKP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SKP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q9WTX5 |
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Standard Curve
| Standard Curve |  |
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