鸡27kDa热休克蛋白(HSPB1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0979
- Price：Negotiable
- Clicks：2489
Product Detail
Product Information
| Product Name | Chicken HSPB1(Heat Shock 27kDa Protein 1) ELISA Kit |
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| Alternative Names | HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein; |
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Assay Parameters
| Species | Chicken |
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| Assay Type | Sandwich |
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| Sensitivity | 0.6 ng/mL |
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| Detection Range | 1.57-100 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Tumor immunity;Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken HSPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HSPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken HSPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken HSPB1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q00649 |
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Standard Curve
| Standard Curve |  |
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