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马肌肉生长抑制素(MSTN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0964
  • Price:Negotiable
  • Clicks:1807

Product Detail

Product Information

Product NameHorse MSTN(Myostatin) ELISA Kit
Alternative NamesGDF8; Growth Differentiation Factor 8

Assay Parameters

SpeciesHorse
Assay TypeSandwich
Sensitivity2.71 ng/mL
Detection Range6.25-400 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Metabolic pathway;Bone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse MSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse MSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse MSTN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9GM97

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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