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大鼠神经激肽A(NKA)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0959
  • Price:Negotiable
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Product Detail

Product Information

Product NameRat NKA(Neurokinin A) ELISA Kit
Alternative NamesNK-A; SK; Substance K; Neuropeptide K; Neurokinin K; Neuromedin L; Neuropeptide gamma

Assay Parameters

SpeciesRat
Assay TypeCompetitive Inhibition
Sensitivity5.34 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2h

Research Information

Research AreaSignal transduction;Neuro science;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NKA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NKA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NKA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP06767

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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