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小鼠PR结构域锌指蛋白16(PRDM16)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0941
  • Price:Negotiable
  • Clicks:1667

Product Detail

Product Information

Product NameMouse PR domain zinc finger protein 16(PRDM16) ELISA Kit
Alternative NamesMEL1; KMT8F; LVNC8; PFM13; CMD1LL

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity6.1 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaNeuro science
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRDM16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRDM16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRDM16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRDM16 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDA2A935

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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