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大鼠游离脂肪酸受体3(FFAR3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0934
  • Price:Negotiable
  • Clicks:1767

Product Detail

Product Information

Product NameRat FFAR3(Free Fatty Acid Receptor 3) ELISA Kit
Alternative NamesFFA3R; GPR41; G Protein-Coupled Receptor 41

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.065 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FFAR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FFAR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FFAR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FFAR3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDB2GV46

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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