小鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0918
- Price：Negotiable
- Clicks：2254
Product Detail
Product Information
| Product Name | Mouse GPC3(Glypican 3) ELISA Kit |
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| Alternative Names | DGSX; OCI5; SDYS; SGB; SGBS1; MXR7; Glypican Proteoglycan 3; GTR2-2; Intestinal protein OCI-5; Secreted glypican-3 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.057 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Tumor immunity;Infection immunity;Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GPC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GPC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GPC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GPC3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q8CFZ4 |
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Standard Curve
| Standard Curve |  |
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