大鼠FK506结合蛋白5(FKBP5)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0879
- Price：Negotiable
- Clicks：2343
Product Detail
Product Information
| Product Name | Rat FKBP5(FK506 Binding Protein 5) ELISA Kit |
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| Alternative Names | P54; FKBP51; FKBP54; Ptg-10; Rotamase; 51 kDa FK506-binding protein; 54 kDa progesterone receptor-associated; Androgen-regulated protein 6; HSP90-binding immunophilin |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.057 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Immune molecule; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FKBP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FKBP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FKBP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FKBP5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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