人乙酰化组蛋白H3(Acetyl-H3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0808
- Price：Negotiable
- Clicks：2425
Product Detail
Product Information
| Product Name | Human Acetyl-H3(Acetyl Histone H3) ELISA Kit |
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| Alternative Names | H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; ; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.16 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Epigenetics and Nuclear Signaling |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P68431 |
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Standard Curve
| Standard Curve |  |
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