大鼠三磷酸肌醇(IP3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0771
- Price：Negotiable
- Clicks：2117
Product Detail
Product Information
| Product Name | Rat IP3(Inositol Triphosphate) ELISA Kit |
|---|---|
| Alternative Names | InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol |
Assay Parameters
| Species | Rat |
|---|---|
| Assay Type | Competitive Inhibition |
| Sensitivity | 5.73 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
Research Information
| Research Area | Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism; |
|---|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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