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山羊三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0744
  • Price:Negotiable
  • Clicks:1746

Product Detail

Product Information

Product NameGoat T3(Triiodothyronine) ELISA Kit
Alternative Names3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Assay Parameters

SpeciesGoat
Assay TypeCompetitive Inhibition
Sensitivity0.048 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2.5h

Research Information

Research AreaEndocrinology;Autoimmunity;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Goat T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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