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大鼠Persephin蛋白(PSPN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0738
  • Price:Negotiable
  • Clicks:2558

Product Detail

Product Information

Product NameRAT PSPN(Presepsin) ELISA Kit
Alternative NamesPSP

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.07 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Infection immunity;Neuro science;Developmental science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO70301

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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