人全反钴胺素(Holo TC)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0720
- Price：Negotiable
- Clicks：2174
Product Detail
Product Information
| Product Name | Human Holo TC(Holotranscobalamin) ELISA Kit |
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| Alternative Names | Holo-TC |
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Assay Parameters
| Species | Human |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 0.045 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 2.5h |
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Research Information
| Research Area | Metabolic pathway;Nutrition metabolism; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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