一步法人单核细胞趋化蛋白1(MCP1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK071ES
- Price：Negotiable
- Clicks：2426
Product Detail
Product Information
| Product Name | EasyStep Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit |
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| Alternative Names | CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.57 pg/mL |
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| Detection Range | 7.81-500 pg/mL |
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| Sample Type | Serum, plasma, cell culture supernatants |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Cytokine;Tumor immunity;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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