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一步法人基质金属蛋白酶8(MMP8)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK070ES
  • Price:Negotiable
  • Clicks:2573

Product Detail

Product Information

Product NameEasyStep Human MMP8(Matrix Metalloproteinase 8) ELISA Kit
Alternative NamesCLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity24 pg/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaEnzyme & Kinase;Infection immunity;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8, and the Human MMP-8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP-8. After TMB substrate solution is added, only those wells that contain Human MMP-8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP-8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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