大鼠成纤维细胞生长因子5(FGF5)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0697
- Price：Negotiable
- Clicks：1743
Product Detail
Product Information
| Product Name | Rat FGF5(Fibroblast Growth Factor 5) ELISA Kit |
|---|
| Alternative Names | HBGF5; Smag82; Heparin-binding growth factor 5 |
|---|
Assay Parameters
| Species | Rat |
|---|
| Assay Type | Sandwich |
|---|
| Sensitivity | 0.075 ng/mL |
|---|
| Detection Range | 0.16-10 ng/mL |
|---|
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
|---|
| Assay Length | 3.5h |
|---|
Research Information
| Research Area | Cytokine; |
|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
| UniProt ID | P48807 |
|---|
Standard Curve
| Standard Curve |  |
|---|
