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大鼠对氧磷酶3(PON3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0695
  • Price:Negotiable
  • Clicks:2384

Product Detail

Product Information

Product NameRat PON3(Paraoxonase 3) ELISA Kit
Alternative NamesSerum paraoxonase/lactonase 3

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.14 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PON3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PON3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PON3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PON3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ68FP2

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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