大鼠丝裂原活化蛋白激酶(MAPK)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0665
- Price：Negotiable
- Clicks：2006
Product Detail
Product Information
| Product Name | Rat MAPK(P38 Mitogen Activated Protein Kinase) ELISA Kit |
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| Alternative Names | P38 Mitogen-Activated Protein Kinase |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.49 ng/mL |
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| Detection Range | 1.25-80 ng/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAPK in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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