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绵羊褪黑素(MT)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0605
  • Price:USD $ 2,800.00
  • Clicks:1952

Product Detail

Product Information

Product NameSheep MT(Melatonin) ELISA Kit
Alternative NamesN-Acetyl-5-Methoxytryptamine

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesSheep
Assay TypeCompetitive Inhibition
Sensitivity5.14 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2.5h

Research Information

Research AreaMetabolic pathway;Tumor immunity;Neuro science;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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