一步法大鼠游离三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK050ES
- Price：Negotiable
- Clicks：2381
Product Detail
Product Information
| Product Name | EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit |
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| Alternative Names | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin |
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Assay Parameters
| Species | Rat |
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| Assay Type | Competitive Inhibition |
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| Sensitivity | 0.31 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | Serum, plasma |
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| Assay Length | 1.5h |
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Research Information
| Research Area | Endocrinology;Autoimmunity; |
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| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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