一步法人游离三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒
- Cat. No.：ELK048ES
- Price：Negotiable
- Clicks：2299
Product Detail
Product Information
| Product Name | EasyStep Human fT3(Free Triiodothyronine) ELISA Kit |
|---|
| Alternative Names | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin |
|---|
Assay Parameters
| Species | Human |
|---|
| Assay Type | Competitive Inhibition |
|---|
| Sensitivity | 0.31 ng/mL |
|---|
| Detection Range | 0.32-20 ng/mL |
|---|
| Sample Type | Serum, plasma |
|---|
| Assay Length | 1.5h |
|---|
Research Information
| Research Area | Endocrinology;Autoimmunity; |
|---|
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT3 antigen, and the Human fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human fT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
Standard Curve
| Standard Curve |  |
|---|
