牛红细胞生成素(EPO)酶联免疫吸附检测试剂盒

Product Name	Cattle EPO(Erythropoietin) ELISA Kit
Alternative Names	EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

Species	Cattle
Assay Type	Sandwich
Sensitivity	29 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Type	serum, plasma, tissue homogenates and other biological fluids
Assay Length	3.5h

Research Area	Cytokine;Endocrinology;Hematology;Hormone metabolism;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle EPO in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P48617

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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