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小鼠角蛋白14(KRT14)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0247
  • Price:USD $ 2,600.00
  • Clicks:2127

Product Detail

Product Information

Product NameMouse KRT14(Keratin 14) ELISA Kit
Alternative NamesCK14; EBS3; EBS4; K14; Cytokeratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin, type I cytoskeletal 14

Specifications & Pricing

48T Price1820
96T Price2600
96T*5 Price11050

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.061 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KRT14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KRT14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KRT14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KRT14 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ61781

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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