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小鼠牙本质基质蛋白1(DMP1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0184
  • Price:Negotiable
  • Clicks:2700

Product Detail

Product Information

Product NameMouse DMP1(Dentin Matrix Protein 1) ELISA Kit
Alternative NamesDMP1 ; Dentin matrix acidic phosphoprotein 1 ; DMP-1; Dentin matrix protein 1

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.067 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates
Assay Length3.5h

Research Information

Research AreaMetabolism
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDO55188

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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